Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: Hormone levels across 12 weeks of resistance training. (A) Total testosterone (TT), (B) sex hormone binding globulin (SHBG) and (C) free androgen index (FAI), (D) DHEA, (E) DHT concentrations. Participants undertaking a 12-week gym-based training program are denoted in blue (n=16), and participants undertaking the blended resistance training program are denoted in red (n=10).

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques: Binding Assay

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: Twelve weeks of resistance training did not affect concentrations of (A) total testosterone (nmol·L -1 ), (B) the free androgen index (FAI; AU), (C) dihydrotestosterone (DHT; pg·mL -1 ) or (D) dehydroepiandrosterone (DHEA; nmol·L -1 ) in plasma of previously untrained, pre-menopausal females (n=27). Data were analysed using a one-way ANOVA. Values are represented as mean ± SD.

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques:

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: The androgen profile measured by LC/MS from urine in pre-menopausal females is not influenced by resistance training. The concentrations of a) testosterone (nmol·L -1 ), b) epitestosterone (nmol·L -1 ), c) androsterone (nmol·L -1 ), d) etiocholanolone (ng·mL -1 ), e) 5α-adiol (ng·mL -1 ), f) 5β-adiol (ng·mL -1 ), g) DHEA (nmol·L -1 ) and h) DHT (nmol·L -1 ) were stable across twelve weeks of resistance training in our cohort (n=27). Data were analysed with one-way ANOVA. Bars indicate mean ± SD.

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet:

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques:

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: Testosterone treatment over 7 days of differentiation increased (A) myotube diameter, (B) AR protein content and (C) AR intensity relative to the proportion of the visual field occupied by myocytes. AR cellular localisation in primary muscle cell lines treated with vehicle (CON) or 100 nM testosterone (TT) after D) 1 day or E) 7 days of treatment (n=6 female donors). Phalloidin (stains actin) appears in blue. AR appears in green. Scale is 0.25 µm·pixel -1 for all images. White scale bar represents 50 µm. Data were analysed using two-tailed, paired t-tests and two-way ANOVA. Values are represented mean ± SD. **indicates p<0.01.

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques: Two Tailed Test

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: Treatment with 100 nM testosterone did not significantly change the expression of A) Akt, B) p-Akt, C) mTOR, D) p-mTOR or E) p-MAPK compared to a vehicle control across 1, 4 or 7 days of differentiation. Black circles represent vehicle control condition (CON), white circles represent testosterone treated (TT) condition. Data were analysed via two-way, repeated measures ANOVA. Bars represent mean ± SD.

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques: Expressing

Journal: Biomedicines

Article Title: Calsarcin-2 May Play a Compensatory Role in the Development of Obese Sarcopenia

doi: 10.3390/biomedicines11102708

Figure Lengend Snippet: Serum calsarcin-2 concentration was negatively associated with skeletal muscle mass index in mice and humans. Eight-week-old C57BL/6J male mice were fed an HFD or chow diet for 24 weeks. The serum calsarcin-2 (CS2) concentrations were determined by enzyme-linked immunosorbent assay, and the total mass of bilateral gastrocnemius muscle mass was measured. The relationship between serum CS2 concentration and skeletal muscle mass per body weight (%) was analyzed using a linear regression analysis. The black dots in the figure represent the assay values for each individual mouse ( A ). A total of seventy-six human subjects were enrolled. The CS2 concentrations were determined by enzyme-linked immunosorbent assay, and the appendicular skeletal muscle mass index (ASMI) was determined by limb skeletal muscle mass (kg)/height 2 (m 2 ). The relationship between serum CS2 concentration and ASMI was analyzed using a linear regression analysis. The black dots in the figure represent the assay values for each individual human ( B ).

Article Snippet: Serum calsarcin-2 concentrations were measured using an enzyme-linked immunosorbent assay kit (intra-assay coefficient of variation (CV) < 10%, inter-assay CV < 12%) (MyBioSource).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay